Sperm Membrane Lipid Profile Differences Between Poor and Good Freezer Stallions A significant subset of stallions exhibits poor sperm tolerance to cryopreservation for reasons that remain incompletely understood. This study used mass spectrometry-based lipidomic analysis to characterize and compare sperm membrane lipid profiles between stallions with established poor and good post-thaw semen quality. Notably, this research emphasizes the… Read More: Sperm membrane lipid profiles in semen from “bad” and “good”… »
Effect of Ozone Gas on Stallion Semen Quality and Microbial Contamination Ozone has demonstrated antimicrobial properties through destabilization of bacterial cell wall phospholipids and inhibition of fungal cell division, however the effect of ozone gas on stallion semen has not been widely evaluated. Given growing interest in reducing antibiotic use in semen extenders, this study evaluated whether ozone… Read More: Effect of ozone gas on stallion semen »
Semen Assessment as a Fertility Predictor in Stallions Accurate stallion semen assessment as a fertility predictor need not rely on expensive equipment. This study investigated whether basic light microscopy assessments of sperm motility, membrane integrity, and morphology could reliably distinguish fertile from sub-fertile stallions under field conditions. Study Design: 24 frozen semen batches from 24 Arabian and Straight… Read More: Semen Assessment as a Fertility Predictor »
Post-Thaw Sperm Motility Comparing Automated Freezer vs. Styrofoam Box Across Multiple Cryoprotectants Standardizing equine semen cryopreservation is challenging due to variability in freezing equipment and cryoprotectant selection. This study compared a computer-controlled automated freezing machine vs. Styrofoam box method across four cryoprotectant combinations to assess post-thaw sperm motility outcomes. Study Design: 4 stallions (ages 4–20); 4 semen collections… Read More: Automated Freezing Machine vs. Styrofoam Box »
Prostanoids as Markers of Oxidative Stress in Stallion Semen Standard pre-processing semen assessment does not reliably predict quality after chilled storage. Oxidative stress in stallion semen during cooling damages sperm membranes via lipid peroxidation, reducing fertilizing potential. This study investigated whether seminal plasma prostanoids — established lipid peroxidation biomarkers — could predict chilled semen quality in stallions. Study… Read More: Prostanoids as Markers of Oxidative Stress in Stallion Semen »
Pre-Freeze Centrifugation Extenders Selection for Optimizing Equine Semen Cryopreservation Cryopreservation exposes spermatozoa to significant physical and chemical stress, making pre-freeze semen centrifugation extender selection critical for maintaining sperm viability and membrane integrity. This study compared four extender formulations — with and without cholesterol and pentoxifylline — to identify the most effective option for pre-centrifugation use in equine semen… Read More: Pre-Freeze Semen Centrifugation Extenders »
Protective Effect of Semen Extenders on Pre-Freeze Shipped Semen During Refrigeration at 5°C for 24 Hours Refrigerating stallion semen at 5°C for 24 hour shipment prior to freezing offers a practical field-based alternative when on-site cryopreservation is not feasible. This study evaluated the effect of semen extenders on pre-freeze shipped semen in four commercial diluents used within a… Read More: Effect of Semen Extenders on Pre-Freeze Shipped Semen »
Increasing Sperm Membrane Cholesterol Improves Cold Shock Resistance and Cryosurvival in Stallions Stallion sperm are inherently sensitive to cold shock due to a low cholesterol-to-phospholipid membrane ratio (0.38–0.45), compared to cold-shock-resistant species such as humans and rabbits (0.88–0.99). This study investigated how much cholesterol supplementation — delivered via cholesterol-loaded cyclodextrins — is required to protect stallion sperm from… Read More: Cholesterol Improves Cold Shock Resistance »
Storage Conditions Affecting Prolonged Storage of Post-thaw Semen Field-based use of cryopreserved stallion semen is often limited by the equipment and expertise required for on-site thawing. Thawing centrally, then achieving prolonged storage of post-thaw semen and shipping it in a cooled state could simplify artificial insemination programs at locations remote from the laboratory or center, but optimal post-thaw… Read More: Prolonged Storage of Post-thaw Semen »
Egg Yolk Concentration, Cryoprotectant Type, and Cooling Rate Effects on Post-Thaw Stallion Sperm Quality Cryopreservation extenders vary widely in egg yolk content and the type of penetrating cryoprotectant used, with prior evidence suggesting amide-based cryoprotectants may reduce oxidative stress compared to glycerol alone. This study examined how egg yolk concentration, cryoprotectant combination, and pre-freeze cooling rate interact to… Read More: Cryopreservation Extenders Content »
