Sperm Kinematic Differences Between Cloned and Non-Cloned Polo Argentino Stallions Cloned horses produced via somatic cell nuclear transfer do not retain the mitochondrial DNA of the founder animal, as mitochondria are derived from the recipient oocyte. Given the established role of mitochondria in sperm motility and energy production, this first-of-its-kind study compared sperm kinematic profiles between cloned and… Read More: Sperm Kinematic Differences Between Cloned and Non-Cloned Stallions »
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Long-Term Liquid Nitrogen Storage Effects on Frozen Stallion Semen Quality and Messenger RNA Abundance Cryopreserved semen stored in liquid nitrogen is widely assumed to maintain fertility indefinitely, yet emerging reports suggest possible long-term quality decline. This study examined whether extended storage duration affects conventional sperm quality parameters and fertility-associated messenger RNA abundance in commercially cryopreserved stallion semen. Study… Read More: Long-Term Storage Effects on Frozen Stallion Semen »
Anti-Müllerian Hormone as a Potential Fertility Biomarker in Stallions Anti-Müllerian hormone, secreted by Sertoli cells, is well-studied in mares but its relationship to stallion fertility remains poorly characterized. Building on evidence linking low serum anti-Müllerian hormone to poor semen quality in infertile men and dogs, this pilot study evaluated whether serum anti-Müllerian hormone concentration could predict breeding performance… Read More: Anti-Müllerian Hormone as a Potential Fertility Biomarker in Stallions »
Sperm Membrane Lipid Profile Differences Between Poor and Good Freezer Stallions A significant subset of stallions exhibits poor sperm tolerance to cryopreservation for reasons that remain incompletely understood. This study used mass spectrometry-based lipidomic analysis to characterize and compare sperm membrane lipid profiles between stallions with established poor and good post-thaw semen quality. Notably, this research emphasizes the… Read More: Sperm membrane lipid profiles in semen from “bad” and “good”… »
Semen Microbiome Influence on the Mare Uterine Microbiome While semen-uterine microbiome interactions have been described in humans, equivalent data in horses was absent. Using a cross-species insemination model, this study investigated whether the male seminal microbiome influences the mare uterine microbiome and whether post-breeding microbiome composition affects embryo recovery. Study Design: 22 fertile mares inseminated with raw semen… Read More: Semen Microbiome Influence on the Mare Uterine Microbiome »
Antimicrobial Resistant Bacteria in Cooled Stallion Semen and Post-Insemination Uterine Lavage Fluid Antibiotic addition to cooled semen extenders is standard practice for bacterial control during storage, yet this may contribute to antimicrobial resistance development and introduce resistant organisms into the mare’s uterus at insemination. This study screened commercial cooled semen and post-insemination uterine lavage fluid for the presence… Read More: Antimicrobial Resistant Bacteria in Cooled Stallion Semen »
Effect of Ozone Gas on Stallion Semen Quality and Microbial Contamination Ozone has demonstrated antimicrobial properties through destabilization of bacterial cell wall phospholipids and inhibition of fungal cell division, however the effect of ozone gas on stallion semen has not been widely evaluated. Given growing interest in reducing antibiotic use in semen extenders, this study evaluated whether ozone… Read More: Effect of ozone gas on stallion semen »
Cryptorchidism in the colt – This article describes the different forms, tests, resolutions and outcomes of cryptorchidism seen in the male horse (colt or stallion). by Jos Mottershead What is Cryptorchidism in the Colt? Before birth, the colt’s testes are located within the abdominal cavity. Testicular descent normally occurs during late gestation or shortly after birth1. Sometimes for… Read More: Cryptorchidism in the Colt »
Semen Assessment as a Fertility Predictor in Stallions Accurate stallion semen assessment as a fertility predictor need not rely on expensive equipment. This study investigated whether basic light microscopy assessments of sperm motility, membrane integrity, and morphology could reliably distinguish fertile from sub-fertile stallions under field conditions. Study Design: 24 frozen semen batches from 24 Arabian and Straight… Read More: Semen Assessment as a Fertility Predictor »