Pre-Freeze Centrifugation Extenders Selection for Optimizing Equine Semen Cryopreservation Cryopreservation exposes spermatozoa to significant physical and chemical stress, making pre-freeze semen centrifugation extender selection critical for maintaining sperm viability and membrane integrity. This study compared four extender formulations — with and without cholesterol and pentoxifylline — to identify the most effective option for pre-centrifugation use in equine semen… Read More: Pre-Freeze Semen Centrifugation Extenders »
Testicular and Epididymal Gene Expression Changes in Response to Scrotal Heating in Stallions Heat stress is a well-recognized cause of testicular and epididymal dysfunction, yet the underlying molecular events driving these changes remain poorly understood. This study used transcriptomic analysis to characterize early gene expression responses in testicular and epididymal tissues following acute scrotal insulation in stallions. Study… Read More: Changes in Response to Scrotal Heating »
Epididymal Sperm Collection and Processing Epididymal sperm collection and processing represent the last opportunity for genetic material rescue following sudden stallion death or emergency castration. This study evaluated multiple collection and processing variables across 142 samples to develop a standardized, practitioner-ready protocol. Study Design: 142 samples collected from stallions undergoing elective orchiectomy (castration) Computer-assisted sperm analysis used for… Read More: Epididymal Sperm Collection and Processing »
Pharmacologically Induced Ejaculation in Stallions Since Drs. McDonnell and Love pioneered research into Pharmacologically Induced Ejaculation in Stallions in the 1990s1, “chemical collection” has been a tool in many advanced stallion manager’s and veterinarian’s collection tool box. The original research produced a tried-and-tested protocol involving Imipramine and Xylazine, and ultimately was considered successful in about 70% of stallions,… Read More: Pharmacologically Induced Ejaculation in Stallions »
Dismount Semen Sample Evaluation, Post-Mating Antibiotics, and Mare Clinical Parameters Background Purpose In Thoroughbred breeding, in-hand mating is the only permitted method, and dismount semen sample evaluations are routinely collected to confirm ejaculation and assess semen quality. Post-mating uterine antibiotic infusions (ceftiofur or ticarcillin) are commonly used to reduce infection risk. This study examined whether dismount sample characteristics… Read More: Dismount Semen Sample Evaluation »
ISSR-8 “Snippets” The 8th International Symposium on Stallion Reproduction Held in Spain in February 2025, ISSR-8 produced a variety of useful research with immediate clinical value. We are honoured to have been permitted to share ISSR-8 presentation “snippets” of the research by – and in association with – the International Society for Equine Reproduction Presentations included a wide… Read More: ISSR-8 »
Processing Equine Semen It’s not difficult to correctly process equine (horse) semen for on-farm insemination or shipping, but it is one about which we often receive questions. Here we look at the basic steps behind achieving a good shipment. This article reviews management of a straightforward stallion. Poor quality semen or that with specific issues – although briefly… Read More: Processing Equine Semen »
One-on-one Horse Breeding Training While we are renowned for offering short courses on the subject of horse breeding worldwide, we not infrequently encounter people either at the courses or through our website who are in need of more specific one-on-one horse breeding training. Perhaps they do not own stallions but want to learn more about the reproductive aspects… Read More: Individual Tuition »
Putting together an INRA (French) AV (the Equine-Reproduction.com way) – A pictorial description of assembling the INRA (French) AV with the technique used at Equine-Reproduction.com, LLC At Equine-Reproduction.com we use a variety of artificial vaginas (“AVs”), but one that we particularly favour and use is the INRA (French model) AV from IMV. We have used this AV to… Read More: Assembling Your New INRA-AV and Preparing for Collection »
The Home-Built Power-pole Breeding Mount or “Phantom” – How to build a phantom mare (breeding mount) for collecting semen from stallions. Detailed plans for a homemade power-pole mount. By Jos Mottershead Building a phantom mare (breeding mount) for stallions can be cheap and easy and we have built a lot of these very simple power-pole breeding mounts (also… Read More: Phantom Plans – Build Your Own Breeding Mount! »
